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Urease Biochemical Tube (for Legionella)
Ready-to-use Urease Biochemical Tubes for rapid, visual detection of Legionella species. Based on urease reaction principle, it differentiates Legionella (negative: yellow/unchanged) from urease-positive bacteria (positive: rose-red) after 24-120 hours incubation. Each box contains 20 pre-sterilized glass ampoules for reliable clinical, environmental, and industrial microbiology testing.
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Product Description
Product Introduction
The Urease Biochemical Tube (for Legionella) is a ready-to-use, pre-sterilized liquid culture medium designed for the biochemical identification of Legionella species, including Legionella pneumophila. Eliminating the need for media preparation, pH adjustment, or sterilization in the laboratory, these glass ampoule-packaged tubes deliver consistent, visual results to support routine Legionella detection in clinical, environmental, and industrial settings.
Intended Use
For biochemical identification and differentiation of Legionella species.
Test Principle
The assay is based on the ability of bacteria to produce the enzyme urease: Urease-positive bacteria hydrolyze urea into ammonia, raising the medium’s pH and turning the phenol red indicator rose-red (positive). Legionella species do not produce urease, so the medium remains yellow or unchanged (negative). This clear color differentiation allows for straightforward identification and differentiation of Legionella from urease-positive organisms such as Proteus mirabilis.
Specifications
Quality Control Performance
Note: Sterility control confirms no growth after 3 days of incubation at 30-35°C.
Procedure and Result Interpretation
Inoculation: Using a sterile loop, transfer a pure culture of the test bacterium into the urease biochemical tube.
Incubation: Incubate aerobically at 36±1°C for 24–120 hours.
Observation: Check the color change of the medium daily.
Interpretation:
- Positive (+): Medium turns rose-red (indicates urease production).
- Negative (-): Medium remains yellow or unchanged (no urease production, consistent with Legionella).
- Blank Control: Uninoculated medium should remain light amber/yellow throughout the incubation period.
Applications
- Clinical Microbiology: Identification of Legionella isolates from patient specimens (e.g., respiratory samples).
- Environmental Monitoring: Detection of Legionella in water systems, cooling towers, and plumbing networks.
- Food and Beverage Testing: Legionella screening in food processing environments.
- Research and Education: Biochemical identification and differentiation training in microbiology laboratories.
Key Advantages
- ✅ Ready-to-Use Format: No media preparation, sterilization, or pH adjustment required, saving lab time.
- ✅ Clear Visual Readout: Distinct color changes eliminate ambiguity in results interpretation.
- ✅ Long Shelf Life: Stable for 1 year when stored at 2-8°C.
- ✅ Rigorous QC Validation: Performance tested with ATCC reference strains to ensure batch-to-batch consistency.
- ✅ Convenient Packaging: Glass ampoules prevent contamination and are easy to handle in routine workflows.
Safety and Precautions
- For in vitro laboratory use only. Not for clinical diagnosis or human/animal administration.
- Handle biological samples with appropriate biosafety precautions (e.g., BSL-2 facilities for Legionella work).
- Do not use if the ampoule is cracked, contaminated, or the medium shows turbidity before use.
- Dispose of all inoculated tubes as biohazard waste in accordance with local regulations.
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