🔹 Intended Use

🔹 Core Product Features

• Enhanced with Liver Tissue: The inclusion of liver pieces provides complex nutrients and growth factors that significantly boost the proliferation of C. chauvoei.
• Optimized for Anaerobes: The formulation maintains a low redox potential, creating the ideal reducing environment required for strict anaerobic bacteria.
• Heat-Stable Formulation: The medium is designed to withstand autoclaving at 116°C for 40 minutes without degradation, ensuring consistent performance.
• High-Yield Performance: Promotes vigorous bacterial growth, resulting in high cell counts suitable for large-scale vaccine production.

🔹 Directions for Use

1. Suspend 27.0 g of the base medium powder in 1000 mL of distilled water.
2. Add an appropriate amount of liver pieces (typically 5-10% v/v) to the solution.
3. Heat to boiling to completely dissolve the powder.
4. Sterilize by autoclaving at 116°C for 40 minutes.
5. Cool to room temperature under anaerobic conditions before use.

🔹 Microbial Sensitivity Test

Test Results

🔹 Product Specifications