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Streptococcus agalactiae Vaccine Medium

Streptococcus agalactiae Vaccine Medium
This specialized medium is formulated for the cultivation of Streptococcus agalactiae (Group B Streptococcus), the causative agent of mastitis in dairy animals. It provides rich nutrients that support robust growth of this fastidious bacterium, making it ideal for high-yield vaccine production.
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Product Description

🔹 Intended Use

For the cultivation of Streptococcus agalactiae for mastitis vaccine manufacturing.

🔹 Core Product Features

  • High Nutrient Density: Formulated with peptones, yeast extract, and other growth factors to support the vigorous growth of fastidious streptococci.
  • pH-Stable Formulation: Buffered to maintain optimal pH levels during growth, ensuring consistent metabolic activity and toxin production.
  • Heat-Stable: Withstands autoclaving at 116°C for 40 minutes without nutrient degradation.
  • Consistent High-Yield Performance: Delivers reliable bacterial counts, supporting the production of high-quality, potent vaccines.

🔹 Directions for Use

  1. Suspend 41.4 g of the medium powder in 1000 mL of distilled water.
  2. Heat to boiling to completely dissolve the powder.
  3. Sterilize by autoclaving at 116°C for 40 minutes.
  4. Cool to room temperature before inoculation.

🔹 Microbial Sensitivity Test

Test Protocol: Inoculate the QC strain and incubate at 36±1°C for 18–24 hours.

Test Results

QC StrainStrain No.9-Tube Method (Growth in Replicates)
Solid Count Method (CFU/mL)


ControlSample
Streptococcus agalactiaeATCC 1381310⁻⁵, 10⁻⁶, 10⁻⁷: 3/3 positive10⁻⁵, 10⁻⁶: 3/3 positive; 10⁻⁷: 2/3 positiveControl: 3.2 × 10⁹ CFU/mLSample: 2.7 × 10⁹ CFU/mL

🔹 Product Specifications

Multiple package specifications are available. Please contact our customer service for detailed product information and purchasing consultation.


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