🔹 Intended Use
For the cultivation of Streptococcus agalactiae for mastitis vaccine manufacturing.
🔹 Core Product Features
- High Nutrient Density: Formulated with peptones, yeast extract, and other growth factors to support the vigorous growth of fastidious streptococci.
- pH-Stable Formulation: Buffered to maintain optimal pH levels during growth, ensuring consistent metabolic activity and toxin production.
- Heat-Stable: Withstands autoclaving at 116°C for 40 minutes without nutrient degradation.
- Consistent High-Yield Performance: Delivers reliable bacterial counts, supporting the production of high-quality, potent vaccines.
🔹 Directions for Use
- Suspend 41.4 g of the medium powder in 1000 mL of distilled water.
- Heat to boiling to completely dissolve the powder.
- Sterilize by autoclaving at 116°C for 40 minutes.
- Cool to room temperature before inoculation.
🔹 Microbial Sensitivity Test
Test Protocol: Inoculate the QC strain and incubate at 36±1°C for 18–24 hours.
Test Results
| QC Strain | Strain No. | 9-Tube Method (Growth in Replicates) |
| Solid Count Method (CFU/mL) |
|---|
|
| Control | Sample |
|
| Streptococcus agalactiae | ATCC 13813 | 10⁻⁵, 10⁻⁶, 10⁻⁷: 3/3 positive | 10⁻⁵, 10⁻⁶: 3/3 positive; 10⁻⁷: 2/3 positive | Control: 3.2 × 10⁹ CFU/mLSample: 2.7 × 10⁹ CFU/mL |
🔹 Product Specifications
Multiple package specifications are available. Please contact our customer service for detailed product information and purchasing consultation.