Sabouraud Dextrose Agar (SDA) Product Description

Product Overview: Sabouraud Dextrose Agar (SDA) serves as a specialized culture medium designed for the isolation, differentiation, and maintenance of fungi, including yeasts and molds. As a result, laboratories frequently use it in pharmaceutical testing, food safety, and clinical microbiology, making it essential for microbial analysis.

Technical Data:

• Approximate Formula:

  • Mycological Peptone: 10.0 g/liter
  • Glucose: 40.0 g/liter
  • Agar: 15.0 g/liter
  • Final pH: 5.6 ± 0.2 at 25°C

• Appearance:

  • Dehydrated Medium: A free-flowing yellowish powder
  • Prepared Medium: A yellowish transparent gel

• Preparation Instructions:

  1. First, suspend 65 g of SDA in 1 liter of distilled or deionized water.
  2. Next, mix well and heat with frequent agitation; then, boil for one minute.
  3. Finally, autoclave the solution at 121°C (15 lbs) for 15 minutes.

• Storage Conditions:

  • Store the tightly capped container at 5-30°C.
  • In addition, the dehydrated medium has a shelf life of three years. For the prepared medium, keep it at 2-8°C, protected from light.

Key Features:

• Effective Growth Environment: The high glucose concentration and low pH create conditions that not only promote fungal growth but also inhibit bacterial contamination.
• Versatile Applications: Consequently, researchers often use SDA for microbial limit tests in pharmaceuticals, food safety, cosmetics, and clinical specimens.
• Quality Control: After incubating at 20-25°C for 48-72 hours, you can observe cultural characteristics to ensure consistent results.

Precautions:

• This product is intended for laboratory use only.
• Furthermore, avoid using the dehydrated medium if it is past its shelf life or shows any signs of caking or color variation.

Conclusion: In summary, Sabouraud Dextrose Agar stands out as a reliable and user-friendly medium crucial for effective fungal culture and identification. Thanks to its optimized formula and straightforward preparation, many laboratories prefer SDA for high-quality microbial analysis. Therefore, equip your lab today with SDA to achieve superior performance in fungal studies.